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At last, a 3 cm section of the colon from The underside to the highest was gathered and divided into three parts. One piece was fastened in a 10% neutral formalin solution and stored for foreseeable future use, and the other two parts have been minced, blended, and divided into two copies, which have been both positioned in cryotubes and temporaril
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The preliminary experiments utilizing the environmentally friendly fluorescence protein-expressing vector uncovered that effectiveness of transfection using this type of program was about 51% with cell viability greater than sixty four%, as calculated by annexin V staining.This function is accredited beneath a Inventive Commons Attribution four.0 I